For Research Use Only. • Melamed MR. Flow Cytometry and Sorting. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. 1997; Gasol and del Giorgio 2000) used since the 1980s (Trask et al. BD Accuri C6 with single tube loader protocol This protocol contains instructions for setting up an Accuri C6 with Tube Loader. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Interest Areas. 1982; Olson et al. This portable and compact system holds the analytical power of a cytometer twice its size. Even new flow cytometry users find it so easy to learn and use that As you probably know, FCS (Flow Cytometry Standard) files are created by flow cytometers. This Cytometer is designed to deliver superior performance with ease of installation and operation for research applications.Cell counters for accurate and timely cell counts of dead cells, live cells and total cells are available at VWR. Two additional features further streamline the process: Absolute counts. 9) Leave the tube on the SIP until the cytometer is used again. DI water, Cat. Prior to measurement, the performance of the Accuri C6 was evaluated by analyzing eight peak rainbow particles (Spherotech, Lake Forest, IL). View online or download Bd Accuri C6 Instrument Manual. Two additional features further streamline the process: Absolute counts. The BD Accuri™ C6 Plus offers simplified workflows and ready-to-use templates for performing a wide range of applications in flow cytometry. Invitrogen Flow Cytometry Size Calibration Kit. If you have questions or require further clarification, see the BD Accuri C6 Flow Cytometer Instrument Manual or contact Accuri Technical Support. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER .....11 3.1 Running Validation Beads (Daily Start-Up) .....11 3.2 Saving Validation Bead Data .....15 3.3 Analyzing and Recording Validation Bead Data.....15 3.4 Monitoring Validation Bead Data.....19 3.5 Troubleshooting Validation .....20 4 MANUAL DATA ACQUISITION.....22 4.1 Viewing the Manual Collect Tab.....22 4.2 … Of the BD Accuri C6 Flow Cytometer Instrument Manual Research Use Only. Preferences in FlowJo (v10+) are set by default to scale the data correctly according to the acquisition display and the format of the file. Sign In. BD Accuri C6 flow cytometer analysis . ... Life Technologies Attune, BD Accuri C6, and MoFlo Astrios will scale data from 6 to 10 decades. BD ACCURI FLOW CYTOMETER (B457) Rules and Guidelines. Flow Cytometry: First Principles. Shut down the BD Accuri C6. Bolts. Life Science; Chemicals; Chromatography; Industries; Fisherbrand; Outlet Corner; Events and Exhibitions. At my lab, we have an Accuri C6 cytometer. 1985) for enumeration and characterization of marine micro-organisms. Nos. tration by as much as 109% when compared to manual count-ing (20). We had a problem with its valves and it was solved in last November. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. For specific questions, you can ask Katie in B457 or email Andrew (BD representative) at tuan_m_nguyen@bd.com. BD Accuri™ personal flow cytometers bring immunology and stem cell studies to the benchtop. BD Accuri cytometers use the C-Flow software, which produces FCS 3.0 compliant files. When combined with the portfolio of BD reagents and best-in-class service and support, it is an ideal solution for life science researchers performing routine assays in cellular analysis, while also reducing operating costs. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Flow cytometry (FCM) is a well-established technique (Marie et al. Learn more Class 1 Laser Product. the flow cytometer Accuri C6 (BD Biosciences, USA) in the clinical chemistry laboratory. New user MUST be trained by the captain or present users. Even more colors to choose from with selectable lasers and filter replacement. If you can simplify cell analysis ITC Data Analysis in versions 8. Before use: 1. Brand: BD | Category: Laboratory Equipment | Size: … BD Accuri C6 Plus software features an intuitive user interface. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Flow Cytometer User Instructions. The reason for this overestimation was probably the different fluorescence patterns affected by the presence of alien particles, and thus a mathematical equation was proposed for correction of values for estimation of sperm concentration (21). BD Accuri C6 flow cytometers, BD CFlow® Software and Workstation PC supply all the capabilities of a full-featured flow cytometer at an affordable price. For more information on general flow cytometry, review the following: • Givan AL. 653154, 653155, and 653157 Between uses Place a tube of DI … BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. For detailed procedures, see the BD Accuri C6 Flow Cytometer Instrument Manual. Take a leap in flow cytometry with the BD Accuri™ C6 Plus. Systems has broken about every year and for core immunophenotyping studies and services from each plot. Notes from the Training Session: • Do not run cells > 40um in diameter (the size of the flow cell is 200um and … Accuri C6 Flow Cytometer Instrument Manual Accuri C6 Software User’s Guide, Chapter 1, The Basics for a complete description of how to run and analyze bead results. BD Accuri C6 Software User Guide - Manual 7820095-01 Rev-0 i TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE.....1 1.1 Starting BD Accuri C6 Software .....1 1.2 BD Accuri C6 Software Workspace .....2 1.3 Opening a New BD Accuri C6 Software Workspace .....2 1.4 Exiting BD Accuri C6 Software.....3 1.5 Using the Example BD Accuri C6 Software File.....3 2 VALIDATING THE … With two lasers, two scatter detectors, and four fluorescence detectors, it can handle most common assays with ease. The Accuri C6 is equipped with two excitation lasers: a blue solid state (488 nm) and a diode red (640 nm) providing up to 6 simultaneous detection pa-rameters, including 4 fluorescent colors plus FSC and SSC. Install and validate the BD Accuri ™ C6 Flow Cytometer with 6- and 8-peak beads prior to installing the BD. 1. 21. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. New York, NY: Wiley-Liss; 1990 (ISBN 0-471-56235-1). Flow Cytometry; Flow Cytometry Supplies; Flow Cytometry Supplies 1 – 30 1132 . New York, NY: Wiley-Liss; 2001 (ISBN 0-471-38224-8). Use only approved fluids, described in the Approved Reagents table of the BD Accuri C6 Flow Cytometer Instrument Manual Yiyi Cao told us why a BD Accuri™ C6 flow cytometer is central to her work, and how it makes flow cytometry accessible to medical students and in the field. Read the interview » Noteworthy Get 40% off dendritic cell reagents. BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer. The cytometer will stop automatically when the time limit is reached. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. Histogram analysis of the forward scatter intensity (FSC) log channel values of the six polystyrene microsphere samples supplied in the Flow Cytometry Size Calibration Kit is shown. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 FL1 — YFP3 488 nm 533/30 FL1 540/20 PE, JC … BD CSampler SIP Collar Tool. Upload. 3rd ed. New York, NY: Alan R. Liss; 1995 Compare the new data to that provided with the C6 and email the file created in Page 4/13. DI water Daily (if the cytometer is not shut down) Run the decontamination and cleaning cycles. Learn to adjust axes scaling factors in over 9. The instrument was validated and maintained daily according to the user manual. All fluorescence signals of labeled spermatozoa were analyzed by the BD Accuri C6 Flow Cytometer and C6 Plus Flow Cytometer (Becton Dickinson, San Jose, CA, USA). 2. It is recommended that the system be shut down if it will not be run again that day. CSampler. The BD Accuri™ C6 personal flow cytometer brings immunology and stem cell studies to the benchtop. Read the Flow Cytometry Size Calibration Kit product manual; Figure 4. • Shapiro H. Practical Flow Cytometry. Inspect the BD CSampler Accessory Kit contents to ensure delivery of all components. With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. Without the BD CSampler installation manual online for full details. The samples were analyzed by an Accuri C6 flow cytometer (BD Biosciences) at 66 µL/min and FL1‐H threshold of 500. With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Can handle most common assays with ease ( B457 ) Rules and.... Technologies Attune, BD Accuri cytometers use the C-Flow software, which produces FCS 3.0 compliant files use! ) Rules and Guidelines available for free PDF download: Instrument Manual research use Only from. 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